高效液相色谱-质谱检测红葡萄皮中花色苷

目的: 建立高效液相色谱-质谱联用(LC-MS)方法,检测红葡萄皮中花色苷。 方法: 采用AichromBond C₁₈色谱柱(250 mm×4.6 mm,5 μm),流动相A(2% 甲酸水溶液)和B(乙腈)梯度洗脱(0 min:10% B;10 min:25% B;15 min:30% B;35 min:50% B)。流速:0.6 mL·min^-1,检测波长:530 nm,二极管阵列检测器,质谱检测设定为负离子模式。 结果: 大泽山玫瑰香红葡萄皮中主要花色苷有15种。 结论: LC-MS法检测红葡萄皮中花色苷,具有高的灵敏度和选择性,方法准确可靠。

Objective: To establish an HPLC-DAD-MS-MS method for determination of anthocyanins from red grape skin. Methods: The major anthocyanins of grape skin was determined by HPLC-DAD-MS-MS method.AichromBond C₁₈ column (250 mm×4.6 mm,5 μm) was used in HPLC assay.Mobile phase A and B were 2% formic acid and acetonitrile,respectively.0 min:10% B;10 min:25% B;15 min:30% B;35 min:50% B.The flow rate was 0.6 mL·min^-1.The detective wavelength was 530 nm.The PDA detector and negative ion mode of MS were adopted. Results: 15 anthocyanins were determined from the red grape skin of Mountain Daze. Conclusion: It is sensitive and selective to determine anthocyanins of grape skin with HPLC-MS method.The method is also accurate and reliable.

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