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期刊名称：药物分析杂志
主管单位：中国科学技术协会
主办单位：中国药学会承办：中国食品药品检定研究院
主编：金少鸿
地址：北京天坛西里2号
邮政编码：100050
电话：010-67012819,67058427 电子邮箱：ywfx@nifdc.org.cn
国际标准刊号：ISSN 0254-1793
国内统一刊号：CN 11-2224/R
邮发代号：2-237
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HPLC法同时测定毛冬青药材中2个三萜皂苷类成分的含量
HPLC simultaneous determination of two triterpene saponins in Ilex pubescens
单位(英文)：1. Chemistry Science and Technology School, Zhanjiang Normal University, Zhanjiang 524048, China;
2. Colege of Chemistry and Chemical Engineering, Guangxi Normal University, Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, Guilin 541004, China;
3. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China
分类号：
出版年·卷·期（页码）：2011,31 (12):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立高效液相色谱法同时测定毛冬青药材中2个三萜皂苷类成分(毛冬青皂苷B1和毛冬青皂苷B2)的含量。 方法: 采用Phenomenex C18(4.6 mm×150 mm,5 μm)色谱柱,以乙腈-0.2%磷酸水溶液为流动相梯度洗脱,流速1.0 mL·min-1,检测波长203 nm。 结果: 毛冬青皂苷B1和毛冬青皂苷B2线性范围分别为2.57～12.82 μg(r=0.9998)和4.51～22.52 μg(r=0.9999),平均回收率(n=6)分别为100.6%(RSD=0.94%)和100.7%(RSD=1.5%)。 结论: 6批样品的测定结果表明,该方法简便、准确,可用于毛冬青药材中毛冬青皂苷B1和毛冬青皂苷B2的含量测定。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To develop an HPLC method for the quantitative determination of two triterpene saponins(ilexsaponin B1 and ilexsaponin B2) in Ilex pubescens. Methods: The determination was carried out on a Phenomenex C18(4.6 mm×150 mm,5 μm) column.The mobile phase was composed of acetonitrile and water(containing 0.2% phosponic acid) with gradient elution.The flow rate was 1 mL·min-1 and the detection wavelength was 203 nm. Results: The linear ranges of ilexsaponin B1 and ilexsaponin B2 were 2.57-12.82 μg(r=0.9998) and 4.51-22.52 μg(r=0.9999),respectively;The average recoveries(n=6) of ilexsaponin B1 and ilexsaponin B2 were 100.6%(RSD=0.94%) and 100.7%(RSD=1.5%),respectively. Conclusion: The determination results of six batch samples show that the method is simple,accurate,and can be used in the quantitative determination of two components(ilexsaponin B1 and ilexsaponin B2) in Ilex pubescens.
-----参考文献：---------------------------------------------------------------------------------------
[1] DENG Liu-qin(邓六勤),ZHONG Ming(钟鸣),MA Zhi-ling(马志玲).Advances in studying on components,pharmacological and clinical applications of Ilex pubescens(毛冬青化学成分、药理作用及临床应用研究进展).Chin Med Mod Dis Educ China(中国中医药现代远程教育),2007,4(10):24
[2] Yamahara J,Hidaka K,Ito M,et al.A triterpene and saponin from roots of Ilex pubescens.Chem Pharm Bull,1987,35(2):524
[3] YANG Yun-yun(杨运云),GUO Peng-ran(郭鹏然),CHEN Yi-lu(陈怡禄),et al.Rapid identification of triterpene and saponin in compound Radix Ilicis Pubescentis medicinal granules by high performance liquid chromatography-mass spectrometry(高效液相色谱-质谱法快速鉴定复方毛冬青冲剂中三萜皂苷活性成分).Chin J Anal Chem(分析化学),2009,37(10):1523
[4] YIN Wen-qing(尹文清),ZHOU Zhong-liu(周中流),ZOU Jie-ming(邹节明),et al.Studies on chemical constituents of pubescent holly root(毛冬青根中化学成分的研究).Chin Tradit Herb Drugs(中草药),2007,38(7):995
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