河北道地药材北沙参HPLC-PDA指纹图谱研究

目的: 建立河北道地药材北沙参HPLC-PDA指纹图谱分析方法,并与不同产地北沙参药材指纹图谱特征进行比较,为科学评价与有效控制北沙参药材质量提供新方法。方法: 采用Diamonsil^TM C₁₈(250 mm×4.6 mm,5μ m)色谱柱,流动相为甲醇-乙腈-0.05%磷酸水溶液,梯度洗脱,流速1.0 mL·min^-1,柱温30 ℃,检测波长295 nm。结果: 13批道地药材的相似度在0.9以上,化学组成一致性较好;不同产地饮片相似度只在0.1~0.4之间。结论: 本方法操作简便、快速、准确,为北沙参药材的鉴别和质量的全面控制提供了依据。

Objective: To establish a fingerprint analysis method for identification of Radix Glehniae of Hebei province, get the control chromatogram and compare the fingerprints of Radix Glehniae collected from different producing areas so as to establish a sensitive and specific method for controlling the quality of Radix Glehniae. Methods: Chromatographic separation was carried out on Diamonsil^TM C₁₈(250 mm×4.6 mm,5 μm) column at 30 ℃. The mobile phase was methanol, acetonitrile and phosphoric acid in aqueous solution(0.05%) with gradient elution. The flow rate of mobile phase was maintained at 1.0 mL·min^-1. The detection wavelength was set at 295 nm. Results: The similarity between 13 batches famous-region drugs were above 0.9, and the chemical composition of them were good consistency. The similarity between other samples from different sources were only 0.1-0.4. Conclusion: This method of the operation is simple, quick, accurate, and can be used for the identification and quality control of Radix Glehniae.