高效液相色谱法测定小鼠血浆头孢匹胺钠的含量

目的: 建立小鼠血浆中头孢匹胺钠HPLC检测方法,为临床上头孢匹胺钠血药浓度监测提供试验依据。 方法: 样品处理采用25%高氯酸(v/v)沉淀蛋白质。PLATISIL^TM C₁₈ (250 mm ×4.6 mm, 5 μm)色谱柱,流动相为甲醇-三乙胺醋酸(三乙胺14 mL ,冰醋酸5. 7 mL ,加蒸馏水定容至100 mL)-水(10∶ 0.2∶ 89.8),1 mol·L^-1醋酸调节pH5.38,流速为0.8 mL·min^-1 ;检测波长为254 nm。 结果: 所建立方法在0.2~250.0 μg·mL^-1范围内线性良好,方法平均回收率为96.1% ,平均提取回收率为77.7%;日内变异RSD小于5%。 小鼠尾静脉给药150 mg·kg^-1 后1 h血浆头孢匹胺钠浓度为(196.1±11.9) μg·mL^-1 ,24 h血药浓度降至(0.5±1.6)μg·mL^-1, 采用DAS2.0程序求得药物动力学参数t_1/2为3.41 h,MRT为3.24 h。 结论: 该法准确、灵敏、快速,内源性成分无干扰,适用于血浆中头孢匹胺钠的分析测定。

Objective: To develop an HPLC method for determination of Cefpiramide Sodium in rat plasma to provide evidence for the monitoring concentration of patients in plasma. Methods: The rat plasma samples were deproteimized with 25% ( v / v) perchlorld acid. A PLATISIL^TM C₁₈ column (250 mm ×4.6 mm, 5 μm) with the mobile phase consisted of methanol- triethylamine acetic acid-water(10∶ 0.2∶ 89.8, v/v), adjusted pH to 5.38 by 1mol·L^-1 acetic acid was used to separate cefpiramide sodium in biological samples, and the detection occurred at 254 nm. Results: A good linearity was obtained in the concentration range of 0.2-250 μg·mL^-1 , the average recovery was 77.7% , the intra-day precision (RSD) was less than 5%. The concentration of cefpiramide sodium in rat plasma was (196.1±11.9) μg·mL^-1 at 1.0 h after iv. 150 mg·kg^-1 cefpiramide sodium, and decreased to (0.5±1.6)μg·mL^-1 at 24 h,The Pharmacokinetic parameters t_1/2 was 3.41 h,MRT was 3.24 h. Conclusion: The described method was proved to be sensitive, rapid and accurate, can be applied in identification and determination of cefpiramide sodium in rat plasma.