三七总皂苷提取工艺的研究

目的: 优选出三七总皂苷提取工艺。 方法: 采用正交设计法安排试验进行优选,以分光光度法测得总皂苷的含量为指标进行评价,利用多元回归分析,确定最佳提取工艺参数。 结果: 选用回流提取法对三七总皂苷进行提取,最佳工艺条件为:选用60%的乙醇,总量为药材的8倍,浸泡40 min,热回流3次,每次1 h。 结论: 三七总皂苷得率高,稳定性好,适合工业生产。

Objective: To study the extraction process for the total saponins in Panax notoginseng (Burk.) F.H.Chen. Method: To set up the experiment by the way of orthogonal design and optimized the selection.Use the total saponins content and total saponins extracting rate,which is measured by the way of sepectrophotometry,as the detective markers to evaluate the experiment.To fix on optimized parameters for extraction process through multiple linear regressions. Results: Indicated the optimum extraction process was adding 8 times amount of 60% alcohol,soaking for 40 minutes,refluxing and extracting for 3 times and 1 hour each time.The extraction process could raise the yield of ontoginseng saponin. Conclusion: The conclusion was this extraction process showed higher yield of notoginseng saponin,good stability and was available for industrial production.