HPLC测定注射用13种维生素中维生素B₆、烟酰胺、D-泛醇的含量

目的: 建立同时测定注射用13种维生素中维生素B₆、烟酰胺、D-泛醇的HPLC法。 方法: 采用Diamonsil (TM) C₁₈(250 mm×4.6 mm,5 μm)为色谱柱,以20 mmol·L^-1磷酸二氢钾(调pH 5.0)－甲醇(88∶ 12)为流动相,流速为1.0 mL·min^-1,检测波长为214 nm,柱温为25 ℃。 结果: 维生素B₆在0.06046~0.2418 mg·mL^-1范围内、烟酰胺在0.3999~1.599 mg·mL^-1范围内,D-泛醇在0.1521~0.6084 mg·mL^-1范围内具有良好线性关系;其回收率分别为100.2%,99.8%,100.4%。 结论: 本方法快速简便,准确可靠,可用于注射用13种维生素中维生素B₆、烟酰胺和D-泛醇的质量控制方法。

Objective: To establish an HPLC method for the simultaneous determination of vitamin B₆,nicotinamide and D-panthenol in 13 vitamins for injection. Method: A Diamonsil C₁₈ (250 mm×4.6 mm,5 μm) was used as separation column at 25 ℃.The mobile phase was 20 mmol·L^-1 KH₂PO₄ solution (adjusted to pH 5.0)-methanol (88∶ 12) at flow rate of 1.0 mL·min^-1.Detection wavelength was set at 214 nm. Results: The linear ranges of vitamin B₆,nicotinamide and D-panthenol were 0.06046-0.2418 mg·mL^-1 (r =0.9997,n=5),0.3999-1.599 mg·mL^-1(r=0.9996,n=5) and 0.1521-0.6084 mg·mL^-1(r=0.9998,n=5),respectively.The average recoveries (n=9) of Vitamin B₆,Nicotinamide and D-panthenol were 100.3%,99.9% and 99.3%,respectively. Conclusion: The method is simple,accurate,and suitable for determination of the three constituents in 13 vitamins for injection.