HPLC法测定八维钙锌片中烟酰胺、维生素B1、B2、B6_的含量
HPLC determination of nicotinamide,vitamin B1,vitamin B2 and vitamin B6 in Baweigaixin tablets
分类号:
出版年·卷·期(页码):2011,31 (6):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
目的: 建立RP-HPLC法测定八维钙锌片中烟酰胺、维生素B1、B2、B6的含量。 方法: 采用Phenomenex C18柱(150 mm×4.6 mm,5 μm);流动相为庚烷磺酸钠溶液(取庚烷磺酸钠0.6 g,加冰醋酸6 mL与三乙胺1 mL,加水至1000 mL,用2 mol·L-1氢氧化钠溶液调节pH至3.80)-甲醇(80∶ 20);紫外检测波长为280 nm;流速为1 mL·min-1。 结果: 烟酰胺的线性范围为26.463~423.40 μg·mL-1(r=0.9999),平均回收率为100.8%,RSD为1.4%(n=9);维生素B1的线性范围为3.90~62.4 μg·mL-1(r=0.9999),平均回收率为100.9%,RSD为1.3%(n=9);维生素B2的线性范围为4.64~74.24 μg·mL-1(r=0.9999),平均回收率为99.7%,RSD为1.8%(n=9);维生素B6的线性范围为4.95~79.28 μg·mL-1(r=0.9999),平均回收率为100.3%,RSD为2.8%(n=9)。 结论: 本方法操作简便,结果准确、可靠,可同时测定八维钙锌片中烟酰胺、维生素B1、B2、B6的含量。
-----英文摘要:---------------------------------------------------------------------------------------
Objective: To establish the method for the determination of nicotinamid,vitamin B1,vitamin B2 and vitamin B6 in compound reserpine tablets. Method: The separation was performed phenomenex C18(150 mm×4.6 mm,5 μm).Heptanesulfonic acid sodium salt-methanol(80∶ 20)as the mobile phase with the flow rate of 1.0 l·min-1,and 280 nm as the detective length. Results: All the results for the standards curve,the average recovery and the separation showed that the method is satisfactory. Conclusion: The method is simple,fast and accurate for analysis of Baweigaixin tablets.
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