HPLC法同时测定更年安软胶囊中维生素B₁_、维生素B₆_的含量

目的: 建立用反相高效液相色谱法同时测定更年安软胶囊中维生素B₁、维生素B₆含量的方法。 方法: 采用C₁₈(250 mm×4.6 mm,5 μm)色谱柱,流动相为甲醇-0.06%庚烷磺酸钠溶液(含1.2%醋酸与0.12%三乙胺)25∶ 75,检测波长275 nm,流速0.8 mL·min^-1 。 结果: 维生素B₁和维生素B₆线性范围分别为16.02~80.10 μg·mL^-1 和15.84~79.20 μg·mL^-1,相关系数r分别为0.9996和0.9997;平均回收率分别为101.2%(RSD=0.5%)和100.1%(RSD=0.9%)。 结论: 该法可同时测定更年安软胶囊中维生素B₁、维生素B₆的含量,方法简便、准确,重复性好。可为制定更年安软胶囊的质量标准和产品质量控制提供依据。

ive Objective: To establish an HPLC method for simultaneous determination vitamin B₁ and vitamin B₆ in gengnianan soft capsules. Methods: C₁₈(250 mm×4.6 mm,5 μm) column was adopted with methanol-0.06% sodium heptanesulforate(containing 1.2% acetic acid and 0.12% triethylamine)(25∶ 75) as the mobile phase at the flow rate of 0.8 mL·min^-1 and the detection wavelength was 275 nm. Results: The linear ranges of vitamin B₁ vitamin B₆ were 16.02-80.10 μg·mL^-1(r=0.9996) and 15.84-79.20 μg·mL^-1( r=0.9997),average recovery was 100.1%(RSD=0.9%). Conclusion: The method is proved to be simple and accurate,reproducible and it can be to used to determination vitamin B₁ and vitamin B₆ simultaneously in Gengnianan soft capsules.Furthermore,it is provides basis for the setting of quality specifications and quality control of Gengnianan soft capsules.