RP-HPLC同时测定黑水缬草药材中缬草烯酸和缬草素含量

目的: 建立同时测定黑水缬草药材中缬草烯酸和缬草素含量的HPLC检测方法。 方法: 采用Diamonsil C₁₈ (250 mm×4.6 mm,5 μm)色谱柱,以甲醇(A)-0.5%磷酸溶液(B)为流动相,梯度洗脱(0~5 min,60%A→70%A;5~22 min,70%A→82%A;22~30 min,82%A→82%A;30~35 min,82%A→90%A),流速1 mL·min^-1 ,采用切换波长法(268 nm检测缬草烯酸,255 nm检测缬草素),柱温35 ℃。 结果: 缬草烯酸、缬草素质量分别在0.0747~1.1952 μg(r=0.9999)和0.0373~0.5971 μg(r=0.9998)范围内线性关系良好;平均加样回收率(n=6)分别为99.8%(RSD=1.3%),100.2(RSD=1.4%)。 结论: 本法操作简便,结果准确,重复性好,为评价黑水缬草药材的质量提供了可靠的分析方法。

Objective: To develop an HPLC method for the determination of valerenic acid and valepotriate in Valeriana amurensis. Methods: Diamonsil C₁₈ (250 mm×4.6 mm,5 μm) column was adopted,the mobile phase consisted of methanol (A)-0.5% H₃PO₄ solution(B) with gradient elution(0-5 min,60%A→70%A;5-22 min,70%A→82%A;22-30 min,82%A→82%A;30-35 min,82%A→90%A)at the flow rate of 1 mL·min^-1.The detecting wavelength was set at 268 nm for valerenic acid and 255 nm for valepotriate,the column temperature was at 35 ℃. Results: The calibration curve was linear in the range of 0.0747-1.1952 μg for valerenic acid(r=0.9999) and 0.0373-0.5971 μg for valepotriate (r=0.9998);The average recoveries(n=6) of valerenic acid and valepotriate were 99.8%(RSD=1.3%) and 100.2(RSD=1.4%),respectively. Conclusion: This HPLC analysis method is simple and reproducible,which can be used for the quality control of Valeriana amurensis.