泽泻特征图谱保留时间重现性的研究

目的: 研究泽泻特征图谱保留时间在不同实验室之间的重现性。 方法: 采用已建立的泽泻高效液相色谱特征图谱条件在不同仪器及不同色谱柱上测定极性不同的化合物,以它们在不同色谱仪(或柱)上的保留时间进行线性回归,并用得到的线性方程对泽泻特征图谱中峰面积大于总峰面积4%的色谱峰的保留时间进行校正。 结果: 通过校正使保留时间的最大绝对误差由12.719 min减小为0.161 min。 结论: 采用该方法可以校正不同实验室间特征图谱保留时间的漂移,提高特征图谱的重现性。

Objective: To study the reproducibility of specific chromatogram retention time. Methods: A method has been developed to improve the reproducibility of retention time on the specific chromatograms of Rhizoma Alismatis Orientalitis by HPLC.Under the chromatographic conditions of Rhizoma Alismatis Orientalitis specific chromatograms,five substances as standards were used to establish the calibration curves.The retention time shift of the specific chromatogram in which peak area percentage is more than 4% was corrected by linear equation. Results: The results show that the absolute error of retention time is diminished from 12.719 min to 0.161 min after correction by standard calibrations. Conclusion :This method is a good way to correct the retention time shift and raise the reproducibility for specific chromatogram obtained from different C₁₈ columns or different HPLC systems,and can be applied in different laboratories conveniently.