GC-MS联用方法检测降压类中成药及保健食品中非法添加的化学药物

目的: 建立气相色谱-质谱联用法(GC-MS)检测降压类中成药及保健食品中非法添加化学药物的方法。 方法: 采用溶剂超声或液-液萃取方式对样品进行提取富集,GC-MS的Scan模式分析 ,采用Rtx-5 ms毛细管色谱柱(30 m×0.25 mm×0.25 μm) ,程序升温:初始温度150 ℃(1 min),8 ℃·min^-1升至250 ℃(5 min),再以12 ℃·min^-1升至280 ℃(15 min),选择EI离子源,溶剂延迟2 min,质谱扫描40~350 amu。 结果: 本方法能够在30~60 min内快速检测降压类中成药及保健食品中是否非法添加尼群地平等7种化学药物,并在2批样品中分别检出了尼群地平和可乐定。 结论: 该法快速﹑准确,专属性强,可用于快速打假中的检测。

Objective: To establish a GC-MS method for determination of chemical drugs added illegally in chinese patent medicine and health food. Methods: Using solvent extraction or liquid-liquid extraction method,to get the enrichment of samples.The GC-MS was set to scan mode.The analytical capillary column was Rtx-5 ms ( 30 m×0.25 mm×0.25 μm ).The oven temperature was set at 150 ℃ (1 min) initially,it was programmed rising by 8 ℃·min^-1 up to 250 ℃(5 min),and then rising by 12 ℃·min^-1 up to 280 ℃(15 min).The EI ion source was selected and the solvent was delayed by 2 min.The MS scanning was set at the range of 40-350 amu. Results: This method can screen 7 kinds of chemical drugs added illegally in Chinese patent medicine and health food within 30-60 min.Nitrendipine and clonidine were detected in two batches of samples. Conclusion: This method is rapid,accurate,and selective.It can be used for determination of drugs added illegally.