RP-HPLC法同时测定马鞭草中木犀草素、芹菜素和山柰酚

目的: 建立HPLC法测定马鞭草药材中木犀草素、芹菜素和山柰酚的含量。 方法: 采用Diamonsil^TM C₁₈(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.05%磷酸水(40∶ 60)为流动相,流速1.0 mL·min^-1,检测波长350 nm,柱温30 ℃。 结果: 木犀草素、芹菜素和山柰酚浓度分别在1.065~21.30,1.146~22.92,1.645~32.91 μg·mL^-1范围内与峰面积均呈良好的线性关系(r=0.9995~0.9999);平均回收率(n=6)分别为98.1%(RSD=1.8%),100.3%(RSD=1.5%),97.1%(RSD=1.7%)。 结论: 本法操作简便、准确,具有良好的重复性,为马鞭草药材的合理应用及质量控制奠定基础。

Objective: To develop an HPLC method to simultaneously determine the contents of luteolin,apigenin and kaempferol in Verbena oficinalis L.. Methods: The separation was carried out on a C₁₈(250 mm×4.6 mm,5 μm) column with the mixture of acetonitrile-water containing 0.05% phosphoric acid(40∶ 60) as a mobile phase at the flow rate of 1.0 mL·min^-1 and detection wavelength was set at 350 nm.The temperature of column was 30 ℃. Results: The linear relationship was good for luteolin,apigenin and kaempferol in the range of 1.065-21.30,1.146-22.92 and 1.645-32.91 μg·mL^-1(r=0.9995-0.9999).The average recoveries(n=6) were 98.1%(RSD=1.8%),100.3%(RSD=1.5%) and 97.1%(RSD=1.7%). Conclusion: This method is rapid,accurate and repeatable and can be used to determine the contents of luteolin,apigenin and kaempferol and control quality of Verbena oficinalis L..