木犀草素在大鼠体内的药动学研究___

Objective:To establish an HPLC method for the determination of luteolin in rat plasma and to study the pharmacokinetics of luteolin in rats.Methods:An Agilent TC-C₁₈(4.6 mm×150 mm,5 μm) column was adopted，the mobile phase was a mixture of acetonitrile and 0.5 mol·L^-1 potassium dihydrogen phosphate solution(40∶60) at the flow rate of 1.0 mL·min^-1 and the UV detector was operated at 280 nm.After oral administration of luteolin(30 mg·kg^-1),the luteolin concentration in rat plasma was detected by HPLC.The pharmacokinetic parameters were calculated by 3P97 program.Results:In rat plasma,a good linearity between peak area ratio of diosmetin to the internal standard and the concentration was found in the range of 20-1200 ng·mL^-1(r=0.9967);the method recoveries were over 96%.The limit of test was 15 ng·mL^-1.The plasma luteolin concentration-time curve could be evaluated by the two compartment model. T_max,C_max and AUC were (0.732±0.13) h, (0691±014) mg·mL^-1 and (0.435±0.12) mg·h·mL^-1, respectively. Conclusion:The developed HPLC method for the pharmacokinetic study of luteolin in rat after oral administration is precise and specific.