LC-MS/MS法同时测定不同产地半枝莲中野黄芩苷和芹菜素含量 ___

Objective:To detect and quantificate scutellarin and apigenin in Herba Scutellariae Barbatae of different districts by LC-MS/MS Methods:Determination of LC system consisted of a Discovery C₁₈ column(250 mm×4.6 mm,5 μm) with acetonitrile and water(0.1% acetic acid) in linear gradient condition.The flow rate was 1 mL·min^-1 and the wavelength of detection was 335 nm.The mass spectrometer was operated under the negative ion mode with the ESI source.The transition of m/z 461/285 and m/z 269/117 was used to qualify scutellarin and apigenin,respectively. Results:The linear ranges of calibration curve of scutellarin and apigenin were 2.6-72.7 mg·L^-1 and 2.3-63.8 mg·L^-1, respectively.By using the established LC-MS/MS methods,12 samples of Herba Scutellariae Barbatae in different districts were analyzed.The results showed that the contents of scutellarin and apigenin were 0.03%-0.39% and 0.02%-0.30%,respectively.The average recoveries(n=5) of scutellarin and apigenin were 99.1% and 97.3%,and the RSDs were 1.4% and 0.7%,respectively.Conclusion:The proposed method is simple and the result is accurate.It can be used for determination of scutellarin and apigenin in Herba Scutellariae Barbatae.