Abstract Objective：To establish a quantitative method for the simultaneous determination of Huangjiangsu A, zingiberensis newsaponin and dioscin, and contrast the contents of three compounds in root of Dioscorea zingiberensis C. H. Wright from different habitats.Method:After refluxed with 70% ethanol, samples were separated by Diamonsil C18 column(250 mm×4.6 mm, 5 μm)；The mobile phase consisted of methanol(A)-water(B) with gradient elution(0-10 min, 78% A;10-13 min, 78% A→90% A;13-45 min, 90% A),and the flow rate was 1.0 mL·min-1.The temperature of drift tube and gas flow rate were 79.0 ℃ and 2.1 L·min-1, respectivelyResults:Huangjiangsu A,zingiberensis newsaponin and dioscin showed good linearity, and the average recoveries were 100.4%, 103.0% and 95.3%, respectively.Conclusion: The method is simple and accurate for the simultaneous analysis of Huangjiangsu A, zingiberensis newsaponin and dioscin in root of Dioscorea zingiberensis.
Key words:root of Dioscorea zingiberensis C. H. Wright;Huangjiangsu A;zingiberensis newsaponin;dioscin;HPLC-ELSD
