HPLC-ELSD同时测定灯银脑通胶囊中白果内酯、银杏内酯A、人参皂苷Rg1、三七皂苷R1的含量___
HPLC-ELSD simultaneous determination of bilobalide, ginkgolide A, notoginsenoside R1 and ginsenoside Rg1 in Dengyin Naotong capsules
分类号：
出版年·卷·期（页码）：2009,29 (4):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
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Abstract Objective: To develop an HPLC-ELSD method for the simultaneous determination of bilobalide, ginkgolide A, notoginsenoside R1 and ginsenoside Rg1 in Dengyin Naotong capsules. Methods: The chromatographic separation was achieved on a Diamonsil C18（200 mm×4.6 mm, 5μm) column with a mobile phase of methanol-water- tetrahydrofuran (25:70:12). The flow rate was 1.0 mL·min-1 and the column temperature was 25 ℃. The drift-tube temperature of ELSD
was 110 ℃, and the flow rate of air was 2.8 L·min-1. Results: The calibration curve was linear within the range of 0.02- 0.2 mg·mL
-1 (r=0.9999) for bilobalide, 0.126- 1.26 mg·mL
-1 (r=0.9999) for ginkgolide A, 0.108- 1.08 mg·mL
-1 (r=0.9995) for notoginsenoside R1 and 0.306-3.06 mg·mL
-1(r=0.9997)for ginsenoside Rg1, respectively. The recoveries(
n=9)
of bilobalide, ginkgolide A, notoginsenoside R1 and ginsenoside Rg1 were 100.0％, 99.9％, 99.8％ and 100.1％, respectively. Conclusion: The method is accurate, sensitive and specific. It’s can be used for quality control
of Dengyin Naotong capsules.
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