一测多评法测定不同来源石韦及其制剂中3个咖啡酰奎宁酸类成分

目的： 采用一测多评法（QAMS）同时测定3种不同来源石韦及其2种复方制剂中绿原酸、新绿原酸和隐绿原酸的含量。方法： 采用高效液相色谱法，使用InertSustain C¹⁸色谱柱（150 mm×4.6 mm，5 μm）；流动相为甲醇-0.1%磷酸水溶液，梯度洗脱；柱温30℃；体积流量1.0 mL·min^-1；检测波长为327 nm。以绿原酸为内参物，计算其与新绿原酸和隐绿原酸的相对校正因子，建立QAMS并测定各样品的含量，与外标法（ESM）测定结果相比较，以验证QAMS的准确性和可行性。结果： 新绿原酸和隐绿原酸的相对校正因子分别为1.108和0.995，在不同仪器和不同色谱柱重现性良好（RSD<5%）。QAMS测得3种石韦样品及其2种复方制剂中绿原酸、新绿原酸和隐绿原酸的含量范围分别为0.593~3.537、0.119~2.497和0.149~2.254 mg·g^-1，与ESM所测结果之间均无显著差异。结论： 建立的QAMS可应用于3种不同来源石韦、复方石韦胶囊和复方石韦片的质量控制。

Objective: To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous determination of chlorogenic acid,neochlorogenic acid and cryptochlorogenic acid in three species of Pyrrosiae Folium and their preparations. Methods: HPLC was performed on a thermostatic Shimadzu InertSustain C¹⁸ column (150 mm×4.6 mm,5 μm) at 30℃,with the mobile phase comprising of methanol-0.1% phosphoric acid at a flow rate of 1.0 mL·min^-1 in a gradient elution manner. The detection wavelength was set at 327 nm. Using chlorogenic acid as the internal reference substance,the relative correction factors (RCFs) of neochlorogenic acid and cryptochlorogenic acid were determined. These three index contents were determined by external standard method(ESM) and QAMS. The results between two methods were compared to verify the accuracy and feasibility of QAMS. Results: The RCFs of neochlorogenic acid and cryptochlorogenic acid were 1.108 and 0.995,respectively,and showed good reproducibility under different instruments and chromatographic columns (RSD<5%). The content ranges of chlorogenic acid,neochlorogenic acid and cryptochlorogenic acid in three species of Pyrrosiae Folium and two preparations by QAMS were 0.593-3.537 mg·g^-1 0.119-2.497 mg·g^-1 and 0.149-2.254 mg·g^-1,respectively. There were no significant difference between QAMS and ESM. Conclusion: The QAMS can be used for the quality control of Pyrrosiae Folium from three species,Fufang Shiwei capsules and Fufang Shiwei tablets.

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