Objective: To establish an HPLC method for simultaneous determination of five components in Herba Artemisiae Anomalae.Methods: The HPLC assay was performed on a Syncronis C18 (250 mm×4.6 mm, 5 μm) column.The mobile phase was methanol-0.2% phosphoric acid (40:60).The flow rate was 1 mL·min-1, the detection wavelength was 260 nm, the column temperature was 25℃ and the injection volume was 10 μL.Results: Five constituents of quercetin, naringin, kaempferol, formononetin and eupatilin were identified by comparing with the spectra of standard substances.The linear relationship of the five components was good within the range (0.062-0.992 μg, 0.031-0.496 μg, 0.034-0.544 μg, 0.022-0.352 μg and 0.027-0.432 μg).The average recoveries of sample addition were in the range of 97.8%-100.6% (RSD<3.0%).The contents of five components in four batches of samples were 0.022%-0.041%, 0.009%-0.024%, 0.011%-0.023%, 0.006%-0.014% and 0.017%-0.033%. Conclusion: The method is simple, accurate and reproducible.It can be used for the determination of five components in Herba Artemisiae Anomalae.
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