RP-HPLC-DAD同时测定复方石韦胶囊中4个黄酮成分的含量

目的：建立RP-HPLC-DAD 方法，同时测定复方石韦胶囊中苦参酮、槐属二氢黄酮G、异黄腐醇、三叶豆紫檀苷4 个黄酮含量。方法：采用C₁₈ 色谱柱（250 mm×4.6 mm，5 μm），以甲醇-水为流动相梯度洗脱，流速1 mL·min^-1，检测波长300 nm。结果：苦参酮、槐属二氢黄酮G、异黄腐醇、三叶豆紫檀苷线性范围分别为0.34~6.84 μg（r= 0.999 3）、0.16~3.27 μg（r= 0.999 5）、0.04~0.85 μg（r= 0.999 1）、0.19~3.70μg（r= 0.999 8）；精密度、重复性良好，RSD 均小于2.0%；在室温条件下供试溶液12 h 内稳定；平均加样回收率（n=6）在99.2%~101.5%（RSD ≤ 2.2%）。3 批样品中上述4 个成分平均含量依次为94.84~98.85、44.73~47.79、11.16~13.26、54.17~58.74 μg·粒^-1。结论：所建立的分析方法可用于复方石韦胶囊中4 个黄酮成分的含量测定。

Objective: To establish a method for simultaneous determination of four flavonoids in Fufang Shiwei capsules. Methods: Analysis was carried out on a Diamonsil C₁₈ column(250 mm×4.6 mm,5 μm)using methanol-water as mobile phase at the flow rate of 1 mL·min^-1 for gradient elution and the detection wavelength was 300 nm. Results: The calibration curves of the four analytes exhibited good linearity over the range of 0.34-6.84 μg(r= 0.999 3)for kurarinone,0.16-3.27 μg(r=0.999 5)for sophoraflavanone G,0.04-0.85 μg(r= 0.999 1)for isoxanthohumol,and 0.19-3.70 μg(r= 0.999 8)for trifolirhizin. The precision and the repeatability were good with RSD value less than 2.0%. The sample solution was stable in 12 h at room temperature. The average

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