一测多评法测定西洋参类保健食品中9个皂苷类成分的含量

目的：建立一测多评法同时测定西洋参类保健食品中9个活性成分（人参皂苷Rg₁、Re、Rg₂、Ro、Rb₁、Rc、Rb₂、Rb₃、Rd）的含量。方法：采用Agilent Zorbax SB-Aq C₁₈色谱柱（4.6 mm&#215;250 mm，5 μm），0.005%甲酸水溶液（A）-0.005%甲酸乙腈溶液（B）为流动相，梯度洗脱（0~20 min，79% A；20~49 min，79% A→66% A；49~65 min，66% A→60% A），柱温30℃，流速为1.0 mL&#183;min^-1，检测波长203 nm。以人参皂苷Rb₁为参照物，建立与其他8个成分的相对校正因子，计算其含量。并与外标法实测值比较差异。结果：在一定的线性范围内，人参皂苷Rb₁与Rg₁、Re、Rg₂、Ro、Rc、Rb₂、Rb₃，Rd的RCF值分别为0.651 3、1.073、1.601、1.333、0.983 0、1.247、1.160、1.347，且在不同实验条件下重现性良好（RSD<5.0%）。外标法与一测多评法测定各成分含量无显著差异。结论：建立的一测多评法准确性高，可用于西洋参保健食品中皂苷类成分的定量分析及质量评价。

Objective: To establish a method for simultaneous determination of nine main active constituents(ginsenosides Rg₁,Re,Rg₂,Ro,Rb₁,Rc,Rb₂,Rb₃ and Rd) in health foods containing American ginseng by quantitative analysis of multi-components by single marker(QAMS).Method: The chromatographic separation was achieved on an Agilent Zorbax SB-Aq C₁₈ column(250 mm&#215;4.6 mm,5 μm) and the mobile phase consisted of solvent A(0.005% formic acid water solution) and solvent B(0.005% formic acid acetonitrile solution) with gradient elution(0-20 min,79%A;20-49 min,79%A→66%A;49-65 min,66%A→60%A).The column temperature was 30.The flow rate was 1.0 mL&#183;min^-1.The UV detection wavelength was set at 203 nm. Ginsenoside Rb₁ was used as the reference,the relative calibration factors of other 8 constituents to ginsenoside Rb₁ were calculated respectively.And the contents of active constituents were calculated based on the relative calibration factors.The results were compared with those obtained by the external standard method. Results: Relative correction factors of ginsenoside Rg₁,Re,Rg₂,Ro,Rc,Rb₂,Rb₃ and Rd with reference to ginsenoside Rb₁ were 0.651 3,1.073,1.601,1.333,0.983 0,1.247,1.160,and 1.347, respectively,and the repeatability was good in different experimental conditions with RSDs below 5%.There was no significant difference between the results determined by QAMS and the external standard method.Conclusion: The established QAMS method is accurate and can be used for quantitative analysis and quality evaluation of health foods containing American ginseng.