基于液质联用技术的苍术炮制前后苍术苷A的尿排泄动力学研究

目的：采用液质联用技术对大鼠灌胃生、麸炒苍术提取物后苍术苷A的尿排泄动力学规律进行研究。方法：将SD大鼠随机分为2组，分别灌胃给予生、麸炒苍术提取物，收集给药前后不同时间段大鼠尿液，采用多反应监测（MRM）模式对尿液中苍术苷A的浓度进行测定。以DAS 3.2.8软件计算排泄动力学参数，绘制累计排泄率以及排泄速率曲线，比较炮制前后尿排泄规律的异同。结果：生、麸炒苍术中苍术苷A的t_1/2分别为（12.88±1.85）h和（12.44±2.41）h，排泄速率峰值分别为（1.32±0.32）μg·h^-1和（1.99±0.59）μg·h^-1，36 h累积排泄量分别占总排泄量的88.55%和90.34%。结论：苍术麸炒后，其主要成分苍术苷A的尿排泄动力学参数t_1/2和k_e值均无显著变化，但总排泄率明显高于生品。

Objective: To study the urinary excretion regulation of atractyloside A in rats after intragastric administration with the extraction of crude and processed Atractylodes lancea by UPLC-MS/MS.Methods: The SD rats were divided into two groups randomly and gavaged with the extraction of crude and processed Atractylodes lancea respectively,then the rats urine was collected before and after administration.The concentrations of atractyloside A in rat urine were measured by multiple reaction monitoring(MRM) model.The excretion parameters were calculated by DAS 3.2.8 software.The cumulative excretion rate curve and excretion velocity curve were drawn to compare the similarities and differences between crude and processed Atractylodes lancea.Results: The excretion half-lifes of atractyloside A in crude and processed Atractylodes lancea were(12.88±1.85) h and (12.44±2.41) h,respectively.The maximum values of excretion rate were(1.32±0.32)μg·h^-1 and(1.99±0.59)μg·h^-1,respectively.36 h cumulative excretion accounted for 88.55% and 90.34% of total excretion,respectively.Conclusions: The t_1/2 and k_e of atractyloside A in rat urine had no significant difference between crude and processed Atractylodes lancea,but the total excretion rate of the processed sample was higher than the crude one.