一测多评法测定白背叶中3个黄酮碳苷成分的含量

目的：建立一测多评法测定白背叶药材中3个黄酮碳苷成分葫芦巴苷Ⅱ、夏佛托苷和异夏佛托苷的含量，并验证其准确性及可行性。方法：采用高效液相色谱法，使用C₁₈色谱柱（4.6 mm×250 mm，5 μm），柱温25℃，流动相为甲醇-0.1%乙酸水溶液，梯度洗脱，流速1.0 mL·min^-1，检测波长336 nm。以葫芦巴苷Ⅱ为内参物，建立其与夏佛托苷和异夏佛托苷的相对校准因子，计算白背叶药材中夏佛托苷、异夏佛托苷的含量，实现一测多评。同时采用外标法测定白背叶药材中3个黄酮碳苷成分的量，比较计算值与测定值之间的差异，以验证一测多评法在测定中的准确性及可行性。结果：在一定线性范围内，葫芦巴苷Ⅱ与夏佛托苷、异夏佛托苷的相对校正因子分别为1.315、1.022。一测多评法测得6批样品中葫芦巴苷Ⅱ的含量范围为0.75~7.51 mg·g^-1，夏佛托苷的含量范围为1.24~7.56 mg·g^-1，异夏佛托苷的含量范围为1.03~5.99 mg·g^-1，与外标法测定结果无显著性差异。结论：所建立的同时测定葫芦巴苷Ⅱ、夏佛托苷和异夏佛托苷含量的一测多评法准确、可行，可用于白背叶药材的定量分析。

Objective:To develop a method for the simultaneous determination of three flavone C-glycosides(vicenin Ⅱ,schaftoside and isoschaftoside)in the leaves of Mallotus apelta(Lour.)Muell.Arg.by quantitative analysis of multi-components by single-marker(QAMS),and to verify accuracy and feasibility of the method.Methods:HPLC was performed on a C₁₈ analytical column(4.6 mm×250 mm,5 μm)at 25℃ with methanol and 0.1% acetic acid as the mobile phase with gradient elution.ViceninⅡ was used as the internal reference substance.The relative correlation factors(RCFs)of schaftoside and isoschaftoside to vicenin Ⅱ were determined.The contents of these three flavone C-glycosides were determined by the external standard method(ESM)and QAMS method.The QAMS method was validated through comparison of the results obtained by the two different methods.Results:RCFs of schaftoside and isoschaftoside to viceninⅡwere 1.315 and 1.022 respectively.The content ranges of vicenin Ⅱ,schaftoside and isoschaftoside by the QAMS method were 0.75-7.51 mg·g^-1,1.24-7.56 mg·g^-1 and 1.03-5.99 mg·g^-1,respectively.The two methods did not show significant difference in assay results and the RCFs were reliable.Conclusion:The QAMS method for simultaneous determination of viceninⅡ,schaftoside and isoschaftoside is feasible,accurate,and can be used to determine three flavone C-glycosides in the leaves of M.apelta.