RP-HPLC法同时测定紫苏子中α-亚麻酸和亚油酸的含量

目的: 建立RP-HPLC法同时测定紫苏子中α-亚麻酸和亚油酸的含量。 方法: 色谱柱为Agilent Eclipse XDB-C₁₈(150 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-水(90∶10),流速1 mL·min^-1,柱温30 ℃,检测波长205 nm。 结果: α-亚麻酸、亚油酸浓度分别在14.45~144.5 μg·mL^-1(r=0.9998)和5.75~57.5 μg·mL^-1(r=0.9999)范围内与峰面积呈良好的线性关系,平均回收率(n=6)分别为100.2%(RSD=2.3%)和101.2%(RSD=1.5%)。 结论: 本法简便、快速、准确,适用于紫苏子及紫苏子油中α-亚麻酸和亚油酸的同时测定。

Objective: To establish an RP-HPLC method for simultaneous determination of α-linolenic acid and linoleic acid in Fructus Perillae. Methods: The separation was carried out on an Agilent Eclipse XDB-C₁₈(150 mm×4.6 mm,5 μm) column with a mixture of acetonitrile-water (90∶10) as the mobile phase at the flow rate of 1.0 mL·min^-1;The column temperature was maintained at 30 ℃ and the detective wavelength was set at 205 nm. Results: The calibration curves of α-linolenic acid and linoleic acid were linear in the ranges of 14.45-144.5 μg·mL^-1(r=0.9998) and 5.75-57.5 μg·mL^-1(r=0.9999),respectively.The average recoveries(n=6) were 100.2%(RSD=2.3%) and 101.2%(RSD=1.5%). Conclusion: The method is simple,rapid and accurate,which is suitable for simultaneous determination of α-linolenic acid and linoleic acid in Fructus Perillae and Perilla oil.

[1] LIU Hong-xu(刘洪旭),CHEN Hai-bin(陈海滨),WU Chun-min(吴春敏).Advances of studies on Perilla frutescens seeds(紫苏子的研究进展).Strait Pharm J(海峡药学),2004,16(4):5
[2] Simopoulos AP.The importance of the ratio of omega-6/omega-3 essential fatty acids.Biomed Pharmacother,2002,(56):365
[3] SONG Ping(宋萍),WANG Qiao-e(王巧娥),DING Ming-yu(丁明玉),et al.Determination of α-linolenic acid in Perilla seed oil by underivatized capillary gas chromatography(非衍生毛细管气相色谱法直接测定紫苏子油中的α-亚麻酸).Chin J Anal Lab(分析试验室),2005,24(10):88
[4] WANG Wei(王威),YAN Xi-ying(闫喜英),LI Zhen-bao(李振宝),et al.Determination of α-linolenic acid in Perilla frutescens (L.) Britt.by GC(紫苏子中α-亚麻酸的气相色谱法测定).Chin J Pharm Anal(药物分析杂志),2000,20(5):316
[5] YUE Xuan-feng(岳宣峰),ZHANG Hai-song(张海松),ZHANG Zhi-qi(张志琪).GC-MS analysis of fatty acids in Radix Ranunculi Ternati(猫爪草中脂肪酸成分的GC-MS分析).Chin J Pharm Anal(药物分析杂志),2007,27(5):665
[6] WANG Xiao-li(王晓丽),YANG Ming-ming(杨明明),CHI Na(迟娜),et al.Determination of the content of fatty acids in Hippophae rhamnoides L.seed oil by HPLC with precolumn derivatization(柱前衍生化-HPLC法同时测定注射用沙棘籽油中脂肪酸的含量).J Shenyang Pharm Univ(沈阳药科大学学报),2010,27(10):808
[7] CAI Li-hua(蔡丽华),DU Xiao-lan(杜小兰),ZHANG Hua-shan(张华山).Analysis of fatty acid as 6-oxy-(isopropionyl ethylenediamine) derivatives by high performance liquid chromatography with fluorescence detection(6-O-异丙酰乙二胺荧光素衍生HPLC分离荧光检测脂肪酸).J Wuhan Univ(Nat Sci Ed)(武汉大学学报理学版),2011,57(1):18
[8] WANG Qiao-e(王巧娥),SONG Ping(宋萍),DING Ming-yu(丁明玉).Determination of α-linolenic acid in Perilla oil by reversed-phase high performance liquid chromatography coupled with evaporative light-scattering detector(反相高效液相色谱-蒸发光散射检测法测定紫苏子油中的α-亚麻酸).Chin J Chromatogr(色谱),2006,24(1):55